Slovakia will be without key duo Milan Skriniar and Stanislav Lobotka for the Euro 2021 play-off semi-final against the Republic of Ireland in Bratislava.
Inter Milan defender Skriniar has tested positive for coronavirus while in camp with his Slovakian teammates ahead of Thursday's crucial clash.
The 25-year-old centre half is the second Inter Milan player to test positive for COVID-19 after teammate Alessandro Bastoni, who was due to face Ireland's U21s for Italy next week.
Slovakia manager Pavel Hapal has also confirmed that midfielder Lobotka will be unavailable to face Stephen Kenny's visitors, with the Napoli player stuck in Italy in quarantine despite testing negative for the virus.
Lobotka, 25, and his teammates are being quarantined in Naples after Napoli duo Piotr Zielinski and Elif Elmas tested positive for the virus.
"Stano has been tested three times with a negative result, but we must do everything we can to replace him," Hapal said.
Fatih Karagumruk midfielder Erik Saba has been called up to the Slovakia squad to replace Lobotka.
Newcastle goalkeeper Martin Dubravka has already been ruled out due to injury.
The Boys in Green will be without injured skipper Seamus Coleman and Nottingham Forest midfielder Harry Arter, with Josh Cullen called up as a late replacement for the latter.
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